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p tfeb s122  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p tfeb s122
    P Tfeb S122, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+tfeb+s122/Phospho-TFEB+(Ser122)+Antibody/pm41044182-383-93-95
    Average 93 stars, based on 26 article reviews
    p tfeb s122 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Knockdown:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    In Vivo:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Transduction:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    shRNA:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Injection:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Limiting Dilution Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Western Blot:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Clonogenic Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Tube Formation Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Expressing:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Passaging:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Control:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Viability Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Reporter Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Marker:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Quantitative RT-PCR:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Phospho-proteomics:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Incubation:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Comparison:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Gene Expression:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Activity Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Fluorescence:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Sequencing:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Dot Blot:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    In Vitro:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Recombinant:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Immunoprecipitation:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Negative Control:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Knock-In:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Mass Spectrometry:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Quantitation Assay:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).

    Protein Quantitation:


    Article Title: SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis
    Article Snippet: 4E-BP1 (#9644 CST; 1:2000), CTSK (#ab19027 Abcam; 1:1000), FLCN (#3697 CST; 1:1000), GAPDH (#5174 CST; 1:1000), GATA3(#5852 CST; 1:500), GPNMB (#38313 CST; 1:1000), GPNMB (Mouse specific) (#90205 CST; 1:1000), Histone-3 (#4499 CST; 1:4000), HNF4A (#3113 CST; 1:500), Keratin 8 (#ab53280 Abcam; 1:1000), LC3 A, B (#12741 CST; 1:2000), MelanA (#ab210546 Abcam; 1:1000), PAX2 (#9666 CST; 1:500), PAX8 (#59019 CST; 1:500), PMEL (#ab137078 Abcam; 1:1000), Phospho-p70 S6 Kinase (Thr389) (#9205 CST; 1:1000), p70 S6 Kinase (#9202 CST; 1:1000), Phospho-S6 Ribosomal Protein (Ser235/236) (#4858 CST; 1:2000), Phospho-4E BP1 (Ser65) (#9451 CST; 1:2000), Phospho-4E BP1 (Thr37/46) (#2855 CST; 1:1000), p-TFEB(S122) (#86843 CST; 1:2000), p-TFEB(S211) (#37681 CST; 1:2000), Pan-Keratin (Type1) (#83957 CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779 CST; 1:4000), TFE3 (#ABE1400 Sigma; 1:4000), TFEB (#4240 CST; 1:2000), WT1 (#83535 CST; 1:1000), Ub (#43124 CST; 1:1000), LAMTOR1 (#8975 CST; 1:1000), RAPTOR (#2280 CST; 1:1000), ATP6V1A (#39517 CST; 1:1000), ATP6V1B1 (#sc-55544 Santa Cruz; 1:500), ATP6V0C (# PA116676 Thermo Fisher; 1:500), ATP6V1C1 (#sc-271077 Santa Cruz; 1:500), ATP6V0D1 (#sc-393322 Santa Cruz; 1:500), ATP6V0D2 (# PA598618 Thermo Fisher; 1:500), ATP6V1G1 (#sc-25333 Santa Cruz; 1:500), ATP6V1H (#sc-166227 Santa Cruz; 1:500), HA-Tag (#3724 CST; 1:1000).



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    Image Search Results


    Journal: iScience

    Article Title: Targeted dephosphorylation of TFEB promotes its nuclear translocation

    doi: 10.1016/j.isci.2024.110432

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-TFEB p-S122 , Cell Signaling Technology , Cat#87932.

    Techniques: Recombinant, Synthesized, Transfection, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Phospho-proteomics, Software